Clone of cells can be a lot of culture, supernatants were collected and a large number of single clone of antibody in vitro. But the law is limited monoclonal antibody in vitro culture, can not exceed a specific concentration of cells, and every day want to change the culture medium. Hybridoma propagation in vivo can overcome these limitations. Hybridoma cell has the genetic characteristics of lymphocytes from the parental generation to the tumor cells. Such as histocompatibility inoculated into syngeneic mice, or mice (athymic nude mice) can not exclude the hybridoma, hybridoma cells began to unlimited reproduction until host death. Produce mouse ascites tumor cells and serum contain a large number of hybridoma monoclonal antibodies secreted by this antibody titers are often higher than the culture supernatant was 100 to 1000 times. The use of immunosuppressive agents, such as pristane, liquid paraffin, anti-lymphocyte serum, can accelerate and promote tumor growth.
Antigen is able to stimulate the body to produce the (specific) immune response, and product antibodies and sensitized lymphocytes in vitro binding to the immune response, and immune effects of the material (specific reaction). There are two basic features of the antigen First, induction of immune responses, that is, immunogenicity, and second, the product and the immune response to react, which is antigenic. Many substances can become antigen, antigen-specific classification can be found in antigen monoclonal antibody preparation, a lot of material can become antigen, in the conventional scientific experiments, scientific research are often selected in each mouse / rat per injection of 10 ~ 50ug of recombinant proteins, peptides coupled, coupling small molecules such as antigen-specific monoclonal antibody.
Animal spleen hundreds of millions of different B-lymphoblastoid cell lines, with the different genes of different B lymphocytes synthesize different antibodies. When the body by antigen stimulation, many epitopes on the antigen molecule to activate B cells each with a different gene. Activated B cells proliferate to form the effect of B cells (plasma cells) and memory B cells, cloning synthesis of a large number of plasma cells and secrete large amounts of antibody molecules distribution to blood, body fluids. If you elect to create a specific antibody plasma cells were cultured, can be obtained by single-cell proliferation and the formation of the cell populations, ie, monoclonal. Monoclonal cells will synthesize an antigen epitope antibody, called monoclonal antibodies.